Select this product for Molecular Innovations name, Human PAI-1 Activity Sandwich ELISA Kit for Non-Plasma Samples and product number, HPAIKT-NP.

This human PAI-1 activity assay is for the quantitative determination of active plasminogen activator inhibitor type 1 (PAI-1) in non-plasma samples including cell culture media or tissue extracts. For research use only. Strip well format. Reagents for up to 96 tests.

Functionally active PAI-1 present in samples reacts with urokinase coated and dried on a microtiter plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected. After appropriate washing steps, monoclonal anti-human PAI-1 primary antibody binds to the captured enzyme. Excess antibody is washed away and bound monoclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase.

TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active PAI-1 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of human PAI-1.

More Details:

Detection Target: PAI-1

Reactivity: Human

Range: 0.125-100 ng/ml

Sensitivity: 0.029ng/ml

At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.

Please note: this product replaces our old product number IHPAIKT-NP (same product, new number).

Click Here to Download a Copy of the Kit Manual

• Preheat a water bath to 37 C.
• Place the frozen plasma sample directly in the water bath.
• Gently agitate the plasma sample every 5-10 minutes to insure even thawing.
• The total thawing time will depend on the frozen sample volume. A 10 ml sample might be thawed completely in 5 minutes, but a large sample could take up to 1 hour.
• The thawed sample should be used immediately or stored at 4 C for up to 24 hours. Liquid plasma should never be kept on ice or stored below 0 C as the cold may cause clotting.
• Plasma should be visually inspected for signs of clotting or precipitation before use in experiments. A coagulation test such as aPTT can also be performed to verify the preservation of the hemostasis system.
• Plasma should always be snap-frozen by placing the tube in dry ice for at least 1 hour. Large containers can be covered in dry ice for even freezing.
• Frozen plasma should be stored at –20 C or colder. Lower temperatures will help preserve labile plasma proteins for longer time periods.

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This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

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