Select this product for Molecular Innovations name, Human PAI-1 Activity ELISA Kit for Non-Plasma Samples and product number, HPAIKT-NP.

This human PAI-1 activity assay is for the quantitative determination of active plasminogen activator inhibitor type 1 (PAI-1) in non-plasma samples including cell culture media or tissue extracts. For research use only. Strip well format. Reagents for up to 96 tests.

Functionally active PAI-1 present in samples reacts with urokinase coated and dried on a microtiter plate. Latent or complexed PAI-1 will not bind to the plate and will not be detected. After appropriate washing steps, monoclonal anti-human PAI-1 primary antibody binds to the captured enzyme. Excess antibody is washed away and bound monoclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase.

TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active PAI-1 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of human PAI-1.

More Details:

Detection Target: PAI-1

Reactivity: Human

Range: 0.125-100 ng/ml

Sensitivity: 0.029ng/ml

At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.

Please note: this product replaces our old product number IHPAIKT-NP (same product, new number).

Click Here to Download a Copy of the Kit Manual

View all citations/references for ELISA Kits

View all citations and product references

This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

Please be aware the image pictured is for illustrative purposes only, and your product packaging and appearance may vary.