Bovine Vasoactive Intestinal Peptide ELISA Kit

Bovine Vasoactive Intestinal Peptide Sandwich ELISA Kit from Innovative Research is intended for the quantitative determination of Bovine Vasoactive Intestinal Peptide in biofluid samples, such as serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. This is a competitive inhibition Sandwich ELISA in strip well format with reagents for up to 96 tests . VIP present in samples reacts with an anti-Bovine VIP monoclonal capture antibody or antigen coated and dried on a microtiter plate. Following this, VIP conjugated to biotin competes with the antigen in the sample to bind the capture antibody or antigen. Unbound conjugate and sample antigen is washed away and bound conjugate is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is inverse proportional to the concentration of active VIP in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Bovine VIP. Detection Target: Bovine Vasoactive Intestinal Peptide Reactivity: Bovine Range: 7.81-500pg/mL Sensitivity: 3.8pg/mL Storage Conditions: Store all components at 4┬░C This Bovine VIP activity assay is for the quantitative determination of Vasoactive Intestinal Peptide in samples including serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.
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This material is sold for in-vitro use only for research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herein are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

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