Bovine Deoxyribonuclease I RNase/Protease Free
IBODNASEPRF100UNBovine Deoxyribonuclease I RNase/Protease Free from Innovative Research has been chromatographically purified to remove RNase and protease. Supplied as a solution at approximately 2 Kunitz units per microliter approximately 1 mg/ml containing 50% glycerol and 1 mM calcium chloride. This is a liquid with a concentration of 2,000 Kunitz units/ml.
This product is suitable for use in techniques requiring digestion of DNA in the recovery of intact RNA or where the integrity of structural proteins or enzymes must be maintained. Applications have included nick translation, DNA mapping, isolation of nuclear RNA and protein, RNA polymerase synthesis of RNA probes and RT-PCR. It is also used in tissue culture work to digest DNA from damaged cells thereby reducing viscosity, and removing membrane bound DNA fragments. Note: Kunitz units as reported by other suppliers can be 2 to 4 times higher. As measured here, one Kunitz unit digests 1 µg of calf thymus DNA in 10 minutes at 37°C in 50 mM Tris, 1 mM Mg2+, 1 mM Ca2+, pH 7.8. Correlation of digestion units with Kunitz units is different for other DNA and buffer systems. Molecular Biology Grade.
More Details:
- Source: Bovine Pancreas
- Purification: chromatographically purified
- Storage Conditions: 2-8°C or -20°C
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These products are supplied for manufacturing, research and laboratory use only. Researchers and laboratory personnel intending to use any of these products for medical investigation on humans are solely responsible for such use and for compliance with the pertinent regulations of the United States Food and Drug Administration (US-FDA) and other regulations. We do not assume liability for damages resulting from the handling, use and/or disposal of these products, from their use in violation of patent or other rights or reliance upon this information.
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