*E. coli HB101 Competent Cells

Select this product for Molecular Innovations name, HB101 Competent Cells and product number, HB101

E.coli HB101 Competent Cells from Innovative Research are an all purpose reagent for general cloning, sub-cloning and plasmid isolation. For a transformation efficiency of at least 1x106 simply thaw a tube of cells on ice, add 1-5 ul of DNA, gently mix and incubate on ice for 2-5 minutes, then spread 50-100 ul onto a pre-warmed culture plate.

Genotype: F–, thi-1, hsdS20 (rB–, mB–), supE44, recA13, ara-14, leuB6, proA2, lacY1, galK2, rpsL20 (strr), xyl-5, mtl-1.

More Details:

• Strain: E.coli
• Format: Frozen Liquid
• Storage: -70C for up to 12 months
  

Additional Information:
For greater transformation efficiencies heat shock at 37C for 1 minute before plating, or heat shock at 42C for 30 seconds then add SOC media and shake at 225 rpm at 37C for 1 hour before plating. Conveniently supplied as a pack of 10 single use 0.1 ml aliquots.

Select this product for Molecular Innovations name, HB101 Competent Cells and product number, HB101