Rat/ChicKen Corticosterone Competitive ELISA Kit from Innovative Research is a highly sensitive competitive enzyme-linked immunoassay (Competitive ELISA) for measuring Corticosterone in serum, plasma and urine. Reagents for up to 96 tests.This Competitive ELISA Kit uses the Competitive-Competitive ELISA principle. The micro Competitive ELISA plate provided in this Kit has been pre-coated with Rat/ChicKen Corticosterone. During the reaction, Rat/ChicKen Corticosterone in samples or Standard competes with a fixed amount of Rat/ChicKen Corticosterone on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat/ChicKen Corticosterone. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ▒ 2 nm. The concentration of Rat/ChicKen Corticosterone in the samples is then determined by comparing the OD of the samples to the standard curve.More Details: Detection Target: Corticosterone Reactivity: Rat/ChicKen Range: 0.39 - 25ng/mL Sensitivity: 0.24ng/mL Storage Conditions: An unopened kit can be stored at 2 - 8 ░C for 1 month unless otherwise specified in the kit documetation. If the kit is not supposed to be used within 1 month, store the components separately upon receipt according to the directions accompanying the kit., At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.For research use only.
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