Select this product for Molecular Innovations name, Immobilized bovine thrombin and product number, BTHROM-I.

Immobilized Bovine Thrombin from Innovative Research is ideal for cleavage of recombinant fusion proteins with an Leu-Val-Pro-Arg/Gly-Ser thrombin recognition sequence. The resin is easily removed by centrifugation or filtration following the reaction. Immobilized bovine thrombin is highly specific, useful in a wide range of reaction buffers and temperatures, and reusable.

More Details:

• Species: Bovine
• Purity: >95% by SDS-PAGE analysis
• Source: Bovine plasma
• Buffer: 0.1M Tris-HCl; 0.15M NaCl; 0.02% Sodium Azide; pH 7.4
• Storage Conditions: 4 C

Immobilized Thrombin Digestion Protocol
1. Make a digestion buffer consisting of 0.05M Tris-HCl, 0.01M CaCl2, pH 8.0.
2. Dissolve 1 mg of your protein sample in 1 ml digestion buffer.
3. Wash 0.05-0.1ml of immobilized thrombin with 3x0.5ml of digestion buffer. Separate the gel from the buffer after each wash by centrifugation or by column. Discard buffer after each wash.
4. Resuspend the washed immobilized thrombin gel in ~0.2ml of digestion buffer then add the resin to your protein sample.
5. Incubate mixture in a shaking water bath for 1-2 hours at 37C, or overnight at 4C, with rapid agitation.
6. Separate the immobilized thrombin gel from the digestion mixture as noted in step 3. Retain supernatant as your thrombin-digested protein sample.

Note: Optimization of immobilized thrombin protocol is required for specific applications. Recommended reaction conditions are pH 7.0 to 9.0 at 37C. The reaction rate will be increased by increasing the enzyme to protein substrate ratio and incubation temperature. For example, a typical digestion of 4 hours to overnight can be achieved using a ratio of 1:25 enzyme to protein substrate, while it is recommended to use 1:10 enzyme to protein substrate for accelerated thrombin digestion (30-60 minutes).

At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.

For research use only.

Looking for a lower price alterative? Check out our Immobilized Human Thrombin at this link Immobilized Human Thrombin

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This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

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