*Rat Total Factor XII ELISA Kit

Select this product for Molecular Innovations name, Rat Coagulation Factor XII Total Antigen ELISA Kit and product number, RFXIIKT-TOT.

Rat Total Factor XII ELISA Kit from Innovative Research is intended for the quantitative determination of total Factor XII and Factor XIIa antigen in rat plasma. Strip well format. Reagents for up to 96 tests.

Rat Factor XII will bind to the affinity purified capture antibody coated on the microtiter plate. Factor XII and XIIa will react with the antibody on the plate. After appropriate washing steps, biotin labeled antirat Factor XII primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm.

• Detection Target: Total Factor XII
• Reactivity: Rat
• Specificity: This assay recognizes natural and recombinant rat Factor XII and Factor XIIa. Pooled normal plasma from dog, rabbit, cyno monkey, rhesus monkey, pig, and sheep were assayed, and no significant cross-reactivity was observed. Pooled normal plasma from human was assayed and minor cross-reactivity was observed. Significant cross-reaction is observed with pooled normal plasma from mouse
• Range: 0.02 - 10 ng/ml
• Sensitivity: 0.011ng/ml
• Storage Conditions: 2-8°C

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