This Human Caspase 3 Sandwich ELISA Kit from Innovative Research is intended for quantitative detection of human Caspase 3 in cell culture supernate sand serum. Strip well format. Reagents for up to 96 tests.
This human Caspase 3 Sandwich ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Caspase 3 has been precoated onto 96-well plates. Standards(Expression system for standard: E. coli, Immunogen sequence: S29-D175 & A183-H277) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Caspase 3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Caspase 3 amount of sample captured in plate.
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Detection Target: Caspase 3
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Uniprot ID: P42574)
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Reactivity: Human
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Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
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Range: 31.2pg/ml-2000pg/ml
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Sensitivity: <10pg/ml
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Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Additional Information:
The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Caspase-3 is a caspase protein that is encoded by the CASP3 gene. This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.; Immunogen sequence: 333
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