This Human Aggrecan Rapid Sandwich ELISA Kit from Innovative Research is intended is a fast Sandwich ELISA kit, this assay takes less than 1.5 hours to detect Human Aggrecan/ACAN with <50pg/ml sensitivity. format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and urine. This is a TMB colorimetric sandwich Sandwich ELISA kit with short assay time and fast experiment set up. Aggrecan/ACAN tissue specificity: Restricted to cartilages. . Strip well format. Reagents for up to 96 tests. This human Aggrecan Sandwich ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Aggrecan has been precoated onto 96-well plates. Standards (NSO, V20-G675) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for Aggrecan is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Aggrecan amount of sample captured in plate. Detection Target: Aggrecan Uniprot ID: P16112) Reactivity: Human Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins. Range: 1.56ng/ml-100ng/ml Sensitivity: <50pg/ml Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Additional Information:
The capture antibody is monoclonal antibody from mouse, the detection antibody is monoclonal antibody from mouse. Expression system for standard: Aggrecan, also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1, is a protein that in humans is encoded by the ACAN gene. It is a critical component for cartilage structure and the function of joints. Aggrecan provides intervertebral disc and cartilage with the ability to resist compressive loads. The localized high concentrations of aggrecan provide the osmotic properties necessary for normal tissue function with the GAGs producing the swelling pressure that counters compressive loads on the tissue. This functional ability is dependent on a high GAG/aggrecan concentration being present in the tissue extracellular matrix. Also, Aggrecan plays an important role in the organization of the extracellular spaces between neurons in the brain.; Immunogen sequence: 419
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