*Human Total Factor X ELISA Kit

Select this product for Molecular Innovations name, Human Coagulation Factor X Total Antigen ELISA Kit and product number, HFXKT-TOT.

This human coagulation Factor X antigen assay is intended for the quantitative determination of total Factor X antigen in human plasma. For research use only. Strip well format. Reagents for up to 96 tests.

Human Factor X will bind to the capture antibody coated on the microtiter plate. Factor X and Xa will react with the antibody on the plate. After appropriate washing steps, polyclonal anti-human Factor X primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody, which is proportional to the total Factor X present in the samples, is reacted with the secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human Factor X. Color development is proportional to the concentration of Factor X in the samples.

More Details:

Detection Target: Factor X

Reactivity: Human

Range: 0.1-50 ng/ml

Sensitivity: 0.094 ng/ml

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Please note: this product replaces our old product number IHFXKT-TOT (same product, new number).

Click Here to Download a SAMPLE Kit Manual