Human Phospholipase A2 Calcium Independent Sandwich ELISA Kit from Innovative Research is intended for the quantitative determination of Human Phospholipase A2, Calcium Independent in biofluid samples, such as serum, plasma and other biological fluids. This is a sandwich Sandwich ELISA in strip well format with reagents for up to 96 tests . iPLA2 present in samples reacts with an anti-Human iPLA2 capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-iPLA2 detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active iPLA2 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Human iPLA2. Detection Target: Human Phospholipase A2, Calcium Independent Reactivity: Human Range: 78.1-5000pg/mL Sensitivity: 32pg/mL Storage Conditions: Store all components at 4┬░C This Human iPLA2 activity assay is for the quantitative determination of Phospholipase A2, Calcium Independent in samples including serum, plasma and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.

• Preheat a water bath to 37 C.
• Place the frozen plasma sample directly in the water bath.
• Gently agitate the plasma sample every 5-10 minutes to insure even thawing.
• The total thawing time will depend on the frozen sample volume. A 10 ml sample might be thawed completely in 5 minutes, but a large sample could take up to 1 hour.
• The thawed sample should be used immediately or stored at 4 C for up to 24 hours. Liquid plasma should never be kept on ice or stored below 0 C as the cold may cause clotting.
• Plasma should be visually inspected for signs of clotting or precipitation before use in experiments. A coagulation test such as aPTT can also be performed to verify the preservation of the hemostasis system.
• Plasma should always be snap-frozen by placing the tube in dry ice for at least 1 hour. Large containers can be covered in dry ice for even freezing.
• Frozen plasma should be stored at –20 C or colder. Lower temperatures will help preserve labile plasma proteins for longer time periods.

This protocol is applicable for plasma containing any anticoagulant of both human and animal origin. While handling serum does not involve the same risk of clotting due to improper handling, we recommend following the above instructions for both plasma and serum for best results

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This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

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