Human Leptin Receptor Recombinant Protein Lyophilized from Innovative Research has been recombinantly produced in CHO cells. This is a Lyophilized protein buffered in with a purity of ? 98% by SDS-PAGE gel and HPLC analyses..
More Details:
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Species: Human
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Target: LEPR
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Purity: ? 98% by SDS-PAGE gel and HPLC analyses.
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Source: CHO cells
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Storage Conditions: The recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted protein is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing the recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Additional Information:
Encoded for, along with leptin, by the obese (ob) gene, leptin receptor is a single-transmembrane-domain protein of the Type I, or Class I, Cytokine Receptor Family. The full length isoform, OB-Rb, is highly expressed in hypothalamic neurons, T cells, and the vascular endometrium, and is thought to be the only isoform capable of transducing intracellular signals. Isoform OB-Ra, which is widely distributed at varying levels of expression, demonstrates weak signal activity and has been implicated in the active transport of leptin across the blood-brain barrier. Through ligand-binding with leptin receptor and the subsequent JAK2/STAT3 signaling cascade, the adipose-derived cytokine leptin functions to suppress appetite and increase thermogenesis. Leptin and leptin receptor have also, more recently, been implicated in the regulation of immune function, reproduction, glucose homeostasis, bone metabolism, wound healing, hematopoiesis, and angiogenesis. Mutations of the ob gene, which can result in leptin resistance and the down-regulation of ligand and/or receptor expression, have been connected to obesity and hypothalamic pituitary function in various in vivo models, including human, mouse, and rat. Recombinant Human Leptin Receptor is a single, glycosylated polypeptide chain of 818 amino acid residues, corresponding to the human OB-Re isoform, and has a calculated molecular weight of approximately 93.5 kDa. As a result of glycosylation, Recombinant Human Leptin Receptor migrates with an apparent molecular mass of approximately 130-150 kDa by SDS-PAGE gel, under reducing and non-reducing conditions.
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