Human EndoG Fragment Recombinant Protein His-Tag and strepII-Tag N-terminal Liquid from Innovative Research has been recombinantly produced in E. coli. This is a Liquid protein buffered in 1X PBS containing 0.1% SDS and 0.02% NaN₃ with a purity of ~95%.

More Details:

• Species: Human
• Target: ENDOG
• Purity: ~95%
• Source: E. coli
• Storage Conditions: Store in working aliquots at -70°C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Additional Information:

The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribo-nuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD (1-3). EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans (2). EndoG also cleaves DNA in vitro (4).

This recombinant protein can be used for WB and ELISA. For research use only. . At Innovative Research we provide reliable, consistent products that deliver reliable, consistent results.

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This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

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