Human DKK-1 Recombinant Protein Lyophilized from Innovative Research has been recombinantly produced in HEK293 cells. This is a Lyophilized protein buffered in with a purity of Greater than 97% by SDS-PAGE gel and HPLC analyses.

Endotoxin level is less than 0.1 ng per and#956;g (1EU/and#956;g)..

More Details:

• Species: Human
• Target: DKK1
• Purity: Greater than 97% by SDS-PAGE gel and HPLC analyses.
  
  Endotoxin level is less than 0.1 ng per and#956;g (1EU/and#956;g).
• Source: HEK293 cells
• Storage Conditions: The lyophilized DKK-1 recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted DKK-1 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing DKK-1 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Additional Information:

DKK-1 is a member of the DKK protein family which also includes DKK-2, DKK-3 and DKK-4. DKK-1 was originally identified as a Xenopus head forming molecule that behaves as an anTagonist for Wnt signaling. Subsequent studies have shown that DKK-1 and DKK-4 play an important regulatory role in the Wnt /and#946;-catenin signaling pathway by forming inhibitory complexes with LDL receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/and#946;catenin signaling system. LPR5 and LPR6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/and#946;catenin signaling cascade. It has been suggested that by inhibiting Wnt/and#946;-catenin signaling, which is essential for posterior patterning in vertebrates, DKK-1 permits anterior development. This notion is supported by the finding that mice deficient of DKK-1 expression lack head formation and die during embryogenesis. Recombinant human DKK-1 expressed in human 293 cells is a 35-40 kDa glycoprotein containing 235 amino-acid residues.

. At Innovative Research we provide reliable, consistent products that deliver reliable, consistent results.

View all citations and product references

This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

Please be aware the image pictured is for illustrative purposes only, and your product packaging and appearance may vary.

No Data Sheets available