Human Myosin Light Chain Kinase Sandwich ELISA Kit from Innovative Research is intended for the quantitative determination of Human Myosin Light Chain Kinase in biofluid samples, such as tissue homogenates, cell lysates and other biological fluids. This is a sandwich Sandwich ELISA in strip well format with reagents for up to 96 tests . MYLK present in samples reacts with an anti-Human MYLK capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-MYLK detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active MYLK in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Human MYLK. Detection Target: Human Myosin Light Chain Kinase Reactivity: Human Range: 0.156-10ng/mL Sensitivity: 0.053ng/mL Storage Conditions: Store all components at 4┬░C This Human MYLK activity assay is for the quantitative determination of Myosin Light Chain Kinase in samples including tissue homogenates, cell lysates and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.
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This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

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