Human Ribosomal Protein L13A Sandwich ELISA Kit from Innovative Research is intended for the quantitative determination of Human Ribosomal Protein L13A in biofluid samples, such as tissue homogenates and other biological fluids. This is a sandwich Sandwich ELISA in strip well format with reagents for up to 96 tests . RPL13A present in samples reacts with an anti-Human RPL13A capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-RPL13A detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active RPL13A in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Human RPL13A. Detection Target: Human Ribosomal Protein L13A Reactivity: Human Range: 0.156-10ng/mL Sensitivity: 0.058ng/mL Storage Conditions: Store all components at 4┬░C This Human RPL13A activity assay is for the quantitative determination of Ribosomal Protein L13A in samples including tissue homogenates and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.
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