Single Domain Antibody-Mediated Blockade of Programmed Death-Ligand 1 on Dendritic Cells Enhances CD8 T-cell Activation and Cytokine Production

Pooled Human AB Serum Plasma Derived Heat Inactivated from Innovative Research was used in the following study:

Single Domain Antibody-Mediated Blockade of Programmed Death-Ligand 1 on Dendritic Cells Enhances CD8 T-cell Activation and Cytokine Production

Katrijn Broos, Quentin Lecocq, Brenda De Keersmaecker, Geert Raes, Jurgen Corthals, Eva Lion, Kris Thielemans, Nick Devoogdt, Marleen Keyaerts, and Karine Breckpot

Vaccines
7 August 2019

“…Dendritic cell [DC] vaccination is extensively studied as a strategy to activate cancer-specific cytotoxic T lymphocytes [CTLs]. To induce potent anti-tumor CTLs three requirements need to be fulfilled. …CD8pos T cells were plated in triplicate in a 96 well round bottom plate at 105 cells per well in 100µL IMDM containing 1% heat-inactivated human AB serum [Innovative Research, Novi, MI, USA], 100 U/mL penicillin, 100 µg/mL streptomycin, 2 mM L-Glutamine and non-essential amino acids [Sigma Aldrich]…”

Related products available from Innovative Research also include:

• Pooled Human AB Serum Plasma Derived Heat Inactivated
• Single Donor Human Whole Blood
• Single Donor Human Plasma (Blood Derived)
• Pooled Human Plasma Apheresis Derived