Coupling Fluorescence-Activated Cell Sorting and Targeted Analysis of Histone Modification Profiles in Primary Human Leukocytes

Coupling Fluorescence-Activated Cell Sorting and Targeted Analysis of Histone Modification Profiles in Primary Human Leukocytes

Posted by Corey Marcath on

Single Donor Human Buffy Coats Leukocytes from Innovative Research was used in the following study:

 

Coupling Fluorescence-Activated Cell Sorting and Targeted Analysis of Histone Modification Profiles in Primary Human Leukocytes

Jeannie M. Camarillo, Suchitra Swaminathan, Nebiyu A. Abshiru, Jacek W. Sikora, Paul M. Thomas, Neil L. Kelleher

Journal of The American Society for Mass Spectrometry
08 July 2019

“…Histone posttranslational modifications (PTMs) are essential for regulating chromatin and maintaining gene expression throughout cell differentiation. Despite the deep level of understanding of immunophenotypic differentiation pathways in hematopoietic cells, few studies have investigated global levels of histone PTMs required for differentiation and maintenance of these distinct cell types. …De-identified donor peripheral blood (PB) specimen was obtained from AllCells (Alameda, CA), Innovative Research (Novi, MI), or as clinical discards from the Clinical Flow Cytometry Laboratory at Northwestern Memorial Hospital…”

 

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  • Tags: Human Blood Cells, Single Donor Human Buffy Coats Leukocytes

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