"... TILs from patients with pancreatic cancer or brain tumor were isolated and expanded in vitro with the addition of IL-2, IL-15 and IL-21 as previously described. Briefly, fresh tumor tissue was cut into 1-2 mm pieces using a sterile scalple, washed twice with cold PBS and cultured in 24-well plates ... with 10% pooled human AB serum (Innovative Research, Novi, MI) ..."
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