International Journal of Clinical and Experimental Pathology, September 2018
"... After 14 and 22 weeks of feeding the high-fat diet, blood was drawn from the abdominal aorta of mice into 3.14% sodium citrate by using a 23-gauge needle. All samples (citrated plasma) were stored at -80°C until analysis. Plasma PAI-1 levels were determined by using a commercial ELISA kit (IMPAIKT-TOT, Innovative Research, Novi, Michigan, USA) ..."
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