Pooled Human Serum from Innovative Research is whole blood derived. The whole blood is collected from donors in an FDA-approved collection center. The blood is allowed to clot and is then processed into serum via centrifugation, and is frozen immediately after processing.
Please note that this product does not allow for donor requests off-the-shelf, but we can create custom pooled lots to your exact specifications upon request. Contact us for details & pricing.
Viral Testing
Each unit is tested for the standard FDA-required viral markers, and found negative for HBsAg, HCV, HIV-1, HIV-2, HIV-1Ag or HIV-1 NAT, ALT, West Nile Virus NAT, Zika NAT and syphilis using FDA-approved methods. When applicable a licensed NAT for Babesia has been performed and found to be non-reactive, if you would like any further testing or analysis performed prior to shipping, please let us know. While we make every effort to ensure the safety of our products, we recommend following universal precautions as laid out the following organizations WHO, CDC and OSHA, handle biological materials as if capable of spreading infectious disease.
Please note - this product replaces our old product number IPLA-SER, Pooled Normal Human Serum (same product, new number).
- Preheat a water bath to 37 C.
- Place the frozen plasma sample directly in the water bath.
- Gently agitate the plasma sample every 5-10 minutes to insure even thawing.
- The total thawing time will depend on the frozen sample volume. A 10 ml sample might be thawed completely in 5 minutes, but a large sample could take up to 1 hour.
- The thawed sample should be used immediately or stored at 4 C for up to 24 hours. Liquid plasma should never be kept on ice or stored below 0 C as the cold may cause clotting.
- Plasma should be visually inspected for signs of clotting or precipitation before use in experiments. A coagulation test such as aPTT can also be performed to verify the preservation of the hemostasis system.
- Plasma should always be snap-frozen by placing the tube in dry ice for at least 1 hour. Large containers can be covered in dry ice for even freezing.
- Frozen plasma should be stored at –20 C or colder. Lower temperatures will help preserve labile plasma proteins for longer time periods.
This protocol is applicable for plasma containing any anticoagulant of both human and animal origin. While handling serum does not involve the same risk of clotting due to improper handling, we recommend following the above instructions for both plasma and serum for best results
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