Hericium erinaceus isolectins recognize mucin-type O-glycans as tumor-associated carbohydrate antigens on the surface of K562 human leukemia cellsSeonghun Kim
Biological Macromolecules, August 2018
" ... Animal blood products and glycoconjugates [GalÎ²(1,3)GalNAc; Neu5AcÎ±(2,3)GalÎ²(1,3)GalNAc; GalÎ²(1,6)GalNAc] were purchased from Innovative Research (Novi, MI, USA) and ... The hemagglutination activity of lectins was determined using the following twofold serial dilution procedure with intact erythrocytes. Lectin-containing solution (50 Î¼L) was mixed with an equal volume of 4% (v/v) formaldehyde-treated porcine red blood cells suspended in PBS in individual wells of a 96-well microtiter plate ... "