Solid-Phase Microextraction Enables Isolation of BRAF V600E Circulating Tumor DNA from Human Plasma for Detection with a Molecular Beacon Loop-Mediated Isothermal Amplification Assay

Solid-Phase Microextraction Enables Isolation of BRAF V600E Circulating Tumor DNA from Human Plasma for Detection with a Molecular Beacon Loop-Mediated Isothermal Amplification Assay

Posted by Corey Marcath on

Pooled Human Plasma Apheresis Derived from Innovative Research was used in the following study:

 

Solid-Phase Microextraction Enables Isolation of BRAF V600E Circulating Tumor DNA from Human Plasma for Detection with a Molecular Beacon Loop-Mediated Isothermal Amplification Assay

Marcelino Varona, Derek R. Eitzmann, Darshna Pagariya, Robbyn K. Anand, and Jared L. Anderson

Analytical Chemistry
17 Jan 2020

”Circulating tumor DNA (ctDNA) is a promising biomarker that can provide a wealth of information regarding the genetic makeup of cancer as well as provide a guide for monitoring treatment. Methods for rapid and accurate profiling of ctDNA are highly desirable in order to obtain the necessary information from this biomarker. However, isolation of ctDNA and its subsequent analysis remains a challenge due to the dependence on expensive and specialized equipment… Pooled human plasma apheresis derived was obtained from Innovative Research (Novi, MI, USA)...”

 

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  • Tags: Human Serum, Pooled Human Plasma Apheresis Derived

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