Pooled Human Plasma (Blood Derived) from Innovative Research was used in the following study:
Xiaobo Xie, Jing Zhao, Miao Lin, Jinlan Zhang, and Yu Xia
Analytical Chemistry
May 15, 2020
Cholesteryl esters (CEs) are lipids synthesized from cholesterol by fatty acids and are the preferred method of transporting and storing cholesterol. Although CEs are composed of only two parts (cholesterol and fatty acyl), they can be quite diverse from one another. Due to their diversity, various diseases have been linked to an imbalance between cholesterol and CEs. Thus, CE profiles are becoming a common biomarker in metabolic disease monitoring because of their role in cholesterol regulation.
Electrospray ionization-tandem mass spectrometry is often used to identify and analyze CEs, however, it is limited by its lack of ability to provide the location of the carbon-carbon double bond (C = C) in fatty acyls. Researchers in this study paired offline 2-acetylpyridine (2-AP) Paterno-Buchi (PB) reaction and reversed-phase liquid chromatography tandem mass spectrometry (RPLC-MS/MS) to create a highly sensitive CE analysis from complex mixtures. The results indicated improved CE analysis at C = C location level over those reported by gas-chromatography MS or acetone PB-MS/MS methods.
