Gail L. McCormick, Travis R. Robbins, Sonia A. Cavigelli, Tracy Langkilde
Journal of Experimental Biology February 2019
"... This assay measures ability of lizard plasma to hold sheep red blood cells (SRBC) in suspension in vitro. We first measured baseline hemagglutination, which is primarily driven by natural antibodies. SRBC (Innovative Research, Novi, MI, USA) were washed with phosphate buffered saline (PBS) and brought to a 2% solution with PBS. Plasma (25 µL) was plated on a 96-well plate randomly with respect to treatment, diluted 1:1 with PBS, and then serially-diluted to 1:64 in a 96-well plate using a multichannel pipette ..."
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