Platelet Factor 4 as a Novel Exosome Marker in MALDI-MS Analysis of Exosomes from Human Serum
Posted by Corey Marcath on
Pooled Human Serum Off The Clot from Innovative Research was used in the following study:
Platelet Factor 4 as a Novel Exosome Marker in MALDI-MS Analysis of Exosomes from Human Serum
Huu-Quang Nguyen, Dabin Lee, Yeoseon Kim, Minseok Paek, Minsun Kim, Kyoung-Soon Jang, Jooyeon Oh, Young-Sun Lee, Jong Eun Yeon, David M. Lubman, and Jeongkwon Kim
Analytical Chemistry
September 24, 2019
“…Exosomes are nanosized vesicles commonly found in biological fluids as a result of a secretion process involving endosomes and multivesicular bodies. The isolation and analysis of exosomes can be useful for noninvasive clinical diagnosis of a variety of human diseases. We investigated the utility of analyzing exosomal proteins, using matrix-assisted laser desorption/ionization combined with Fourier transform ion cyclotron resonance mass spectrometry (MALDI-FTICR-MS), as a means of determining the presence of exosomes. MALDI-FTICR-MS analyses of exosomes enriched from human serum via centrifugation in a mass range of m/z 1000−20 000 yielded a distinctive protein around m/z 7766. …Pooled normal human serum samples were obtained from Innovative Research (Novi, MI)... “
Related products available from Innovative Research also include:
- Pooled Human Serum Off The Clot
- Single Donor Human Serum Off The Clot
- Pooled Human Complement Serum
- Pooled Human AB Serum Plasma Derived