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Plasminogen activator system homeostasis and its dysregulation by ethanol in astrocyte cultures and the developing brainClare J. Wilhelm, Joel G. Hashimoto, Melissa L. Roberts, Xiaolu Zhang, Calla M. Goeke, Shelley H. Bloom, Marina Guizzetti
Neuropharmacology: Volume 138, August 2018, Pages 193-209
... Levels of tPA were determined in lysates from rat cortices, hippocampi, and mixed-sex astrocyte cultures using a tPA enzyme-linked immunosorbent assay (ELISA) kit (Innovative Research, Novi, MI) as described in the manufacturer's protocol. Levels of PAI-1 were determined in lysates from rat cortices and mixed-sex astrocyte cultures using a PAI-1 ELISA kit (Innovative Research, Novi, MI) as described in the manufacturer's protocol. tPA and PAI-1 concentrations in the samples were interpolated from their respective standard curves and normalized to total protein content...
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