Photooxidation-Induced Fluorescence Amplification System for an Ultra-Sensitive Enzyme-Linked Immunosorbent Assay (ELISA)
Posted by Adam Awdish on
Pooled Human Plasma Apheresis Derived from Innovative Research was used in the following study:
Youhee Heo, Kwanwoo Shin, Min Cheol Park, and Ji Yoon Kang
Scientific Reports
March 12, 2021
It’s important to be able to detect biomarkers, such as proteins or nucleic acids, at very low concentrations in various bodily fluids when attempting to diagnose diseases. There has been a lot of contemporary research looking to identify other disease biomarkers, particularly ones detectable in blood, and reliable methods for detecting said biomarkers with low cost and high sensitivity. Unfortunately, ELISA machines commonly used in diagnostic settings typically cannot detect rare biomarkers, and when they can it’s usually at a much higher concentration threshold than desired. Multiple methods for improving upon the ELISA’s shortcomings have been tested, however, they all fell short due to high cost, difficult procedures, complicated training requirements, or the need for specialized equipment.
In this study, researchers developed a method of photooxidation-induced fluorescence amplification (PIFA) that can be easily implemented on a conventional sandwich ELISA and amplifies small fluorescent signals to measurable levels. In theory, a single molecule can be detected by a PIFA assay, something that conventional HRP-based fluorescence assays cannot do. After being combined with an ELISA kit, the researchers verified that the PIFA assay enhanced detection sensibility by a factor of over 10. This suggests that the new PIFA assay and ELISA kit combination may prove useful in detecting and diagnosing diseases with rare biomarkers.
Related products available from Innovative Research also include:
Pooled Human Serum Off The Clot