Posted by Adam Awdish on
Innovative Grade US Origin Mouse Balb C Serum from Innovative Research was used in the following study:
Adam J. Replogle, Christopher Sexton, John Young, Luke C. Kingry, Martin E. Schriefer, Marc Dolan, Tammi L. Johnson, Neeta P. Connally, Kerry A. Padgett & Jeannine M. Petersen
January 21, 2021
When looking for proof of bacterial infection in a patient, primary culture recovery is the most common procedure to confirm an infection. Despite the rise in the number of infections over the last decade and the identification of new species causing them, isolation of borreliae spp. has proven extremely difficult to isolate in vitro and has rarely been accomplished. This is due to a variety of reasons, including strict requirements for exogenous nutrients and cofactors, lengthy doubling times in vitro, the complex nature of the culture medium required for isolation, and so on.
There are two groups of borreliae that cause illnesses in humans, the Borrelia burgdorferi sensu lato (Bbsl) complex and the relapsing fever (RF) group. Lyme borreliosis (LB) is caused by infection by spirochetes in the Bbsl genospecies complex and is transmitted through hard tick bites, whereas RF develops following infection from spirochetes in the RF group. Over the recent decades, dozens of new species of borreliae have been discovered which are capable of infecting humans.
First found in Japan in 1995, then later identified as a human pathogen in Russia in 2011 and in the U.S. in 2015, Borrelia miyamotoi is one of the new species of borreliae that has proven difficult to isolate in vitro. In this study, researchers created a broadly encompassing borreliae media that can collect 16 different B. miyamotoi isolates from a sample of whole blood in an efficient manner. This discovery can help with in vitro culture recovery of RF and LB spirochetes, creating the possibility for isolating more species of borreliae and other human pathogens.
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