Isolation of Borrelia miyamotoi and Other Borreliae Using a Modified BSK Medium

Isolation of Borrelia miyamotoi and Other Borreliae Using a Modified BSK Medium

Posted by Adam Awdish on

Innovative Grade US Origin Mouse Balb C Serum from Innovative Research was used in the following study:

 

Isolation of Borrelia miyamotoi and Other Borreliae Using a Modified BSK Medium

Adam J. Replogle, Christopher Sexton, John Young, Luke C. Kingry, Martin E. Schriefer, Marc Dolan, Tammi L. Johnson, Neeta P. Connally, Kerry A. Padgett & Jeannine M. Petersen

Scientific Reports
January 21, 2021

When looking for proof of bacterial infection in a patient, primary culture recovery is the most common procedure to confirm an infection. Despite the rise in the number of infections over the last decade and the identification of new species causing them, isolation of borreliae spp. has proven extremely difficult to isolate in vitro and has rarely been accomplished. This is due to a variety of reasons, including strict requirements for exogenous nutrients and cofactors, lengthy doubling times in vitro, the complex nature of the culture medium required for isolation, and so on.

There are two groups of borreliae that cause illnesses in humans, the Borrelia burgdorferi sensu lato (Bbsl) complex and the relapsing fever (RF) group. Lyme borreliosis (LB) is caused by infection by spirochetes in the Bbsl genospecies complex and is transmitted through hard tick bites, whereas RF develops following infection from spirochetes in the RF group. Over the recent decades, dozens of new species of borreliae have been discovered which are capable of infecting humans.

First found in Japan in 1995, then later identified as a human pathogen in Russia in 2011 and in the U.S. in 2015, Borrelia miyamotoi is one of the new species of borreliae that has proven difficult to isolate in vitro. In this study, researchers created a broadly encompassing borreliae media that can collect 16 different B. miyamotoi isolates from a sample of whole blood in an efficient manner. This discovery can help with in vitro culture recovery of RF and LB spirochetes, creating the possibility for isolating more species of borreliae and other human pathogens.

 

Related products available from Innovative Research also include:

Single Donor Human Whole Blood

Pooled Human Plasma (Blood Derived)

Pooled Human Complement Serum

  • Tags: Animal Serum, Innovative Grade US Origin Mouse Balb C Serum, Mouse Balb C Serum

    • The next big thing: it's what you do. The biological research materials you need to discover it? That's what we do! We love seeing how our products are being used in real-life applications and contributing to scientific acheivements every day. With thousands of published references and decades of proven results, you can count on us to help you source the high-quality biomaterials you need that will deliver reliable, consistent results - kit to kit, lot to lot, all study long. So what are you waiting for? All great discoveries start somewhere... yours starts with Innovative Research! Get in touch with our team today!


    Related Posts

    Wide-Targeted Metabolome Analysis Identifies Potential Biomarkers for Prognosis Prediction of Epithelial Ovarian Cancer
    Pooled Human Plasma (Blood Derived) from Innovative Research was used in the following ...
    Read the Article
    In Vitro Validation Assessment of a Fecal Occult Blood Protein Test for Horses
    Horse Albumin Purified Lyophilized from Innovative Research was used in the following s...
    Read the Article
    Self-Assembling Peptide Solution Accelerates Hemostasis
    Innovative Grade US Origin Rat Wistar Whole Blood, Rat Wistar Red Blood Cells, Innovati...
    Read the Article

    ← Older Post Newer Post →

    ×