Posted by Adam Awdish on
Innovative Grade US Origin Monkey Rhesus Whole Blood from Innovative Research was used in the following study:
Madhumithra Subramanian Karthikesh, Sa Wu, Rohit Singh, Yannis Paulus, Xueding Wang and Xinmai Yang
March 3, 2022
Photo-mediated ultrasound therapy (PUT) is a form of non-invasive imaging technique that uses synchronized ultrasound bursts and laser pulses to produce a photoacoustic image and seems to be very precise in blood vessel targeting. Further, PUT has been shown to cause limited damage to healthy tissues surrounding the area of treatment. PUT works by enhancing cavitation activity within the targeted vessels, inducing strong mechanical stresses. These stresses impact the function of platelets, red blood cells, and endothelial cells in blood vessels. Said changes may cause coagulation, hemorrhaging, and vasoconstriction.
The effects of ultrasound treatments on vascular endothelial cells are of particular interest due to the powerful physiological effects endothelial cells have on the body. Thus, the impact of ultrasound on endothelial cells has been studied for possible applications in the fields of drug delivery, gene therapy, injury recovery, DNA and protein synthesis, cancer treatment, and more.
Nitric oxide (NO) helps to regulate many functions in the body, and it can be released when changes in blood flow cause endothelial stress. Along with NO, prostacyclin (PGI2) is released from endothelial cells and, together, they form a specific association that helps in the regulation of vascular and platelet function.
In this study, researchers developed a novel vascular model based on the polydimethylsiloxane (PDMS) channel. PDMS is safe, stable, biocompatible, and hemocompatible, and is widely used in developing in vitro blood vessel models. The newly-developed model has a biologically inert surface to prevent microbial growth and make it ideal for biomedical applications, and is also very permeable to gases, making it ideal for in vitro cell cultivation. Further, the channel is coated with type I collagen, fibrin, or fibronectin to promote cellular adhesion. Previous in vitro studies of endothelial cells involved treating them in Petri dishes or well plates, which are not ideal representations of in vivo models. This new model is aimed at creating a more accurate representation of the changes in PGI2 and NO released from vascular endothelial cells during PUT.
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