Daniel Klapak, Sarah Broadfoot, Gregory Penner Anup Singh, Eshwar Inapuri
PLOS|ONE, October 2018
"... LDL particles were isolated from normal pooled human serum (Innovative Research, Novi, MI) using an established ultracentrifugation protocol . Briefly, three different density solutions (1.006 g/mL, 1.02 g/mL and 1.065 g/mL) of KBR were prepared to enable the separation of the two layers of LDL (LF- and SD-LDL), which fall at the junction between each layer. The visualization (Fig 1) of the LDL heterogeneity was facilitated by prestaining the serum ..."
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