Detection of SARS-CoV-2 RNA through Tandem Isothermal Gene Amplification without Reverse Transcription
Posted by Adam Awdish on
Pooled Human Plasma (Blood Derived) from Innovative Research was used in the following study:
Hyojin Lee, Hyobeen Lee, Sang-Hyun Hwang, Woong Jeong, Dong-Eun Kim
Analytica Chimica Acta
May 3, 2022
It has become clear over the last few years that, to stop the continuous spread of COVID-19 globally, rapid and accurate tests for the detection of SARS-CoV-2 must be developed. Nucleic acid testing is currently considered to be the best method for diagnosing SARS-CoV-2, however, the methods required to carry out the necessary testing, which are commonly PCR-based, can be costly and must be done by specially trained experts in a lab, making them less than ideal for point-of-care (POC) testing.
In attempt to overcome these limitations, methods of isothermal amplification have been developed as alternatives to PCR-based technologies including LAMP, RPA, SDA, and RCA among others. Isothermal amplification is an attractive method for POC testing; however, different methods of isothermal amplification have different limitations, and thus there is a necessity to combine the methods to enable isothermal amplification of viral RNA present in samples at a POC setting.
In this study, researchers developed an isothermal amplification system intended to detect viral SARS-CoV-2 RNA in POC settings. Their methodology combines isothermal gene amplification tools without reverse transcription of RNA into cDNA. Their fluorometric isothermal viral RNA detection system can quantitatively detect long SARS-CoV-2 RNA in a sample with high sensitivity and accuracy and is convenient enough to be used in a POC setting for diagnosis and potentially improved alternative to current PCR-based testing.
Related products available from Innovative Research also include:
Single Donor Human Plasma (Blood Derived)