Posted by Leanne Kodsmann on
Human Complement from Innovative Research was used in the following study:
Tianjiao Duan, Alex J. Smith and Alan S. Verkman
Journal of Neuroinflammation, October 2018
"... Specified concentrations of AQP4-IgG (or control human IgG, Thermo Fisher Scientific, Rockford, IL) and human complement (Innovative Research, Novi, MI) were added in Hank’s buffer, and cells were incubated at 37 °C for specified times. In some experiments, cells were exposed to serum of an AQP4-IgG seropositive NMO patient who met the revised diagnostic criteria for clinical disease. A fixable dead-cell stain (amine-reactive dye, Invitrogen, Eugene, OR) at 1:1000 dilution was added 30 min prior to cell fixation. In some experiments, C1q- or C6-deficient human complement (Innovative Research, Novi, MI) was used instead of normal complement ..."
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