Measuring VACV and ACV in Mouse and Human Plasma

Measuring VACV and ACV in Mouse and Human Plasma

Posted by Leanne Kodsman on

New research published in AAPS PharmSciTech looks at creating a nanodelivery system containing a mucoadhesive polymer hyaluronic acid for oral delivery, using Metformin as the model drug.


Did you know? For this study, Single Donor Human Plasma was obtained commercially from Innovative Research Inc. We love seeing how our products are being used in real-life applications and contributing to scientific achievements!

Pharmacokinetic (PK) studies can be challenging at times, especially when performed on mice where biological sample volumes are limited by things like the size of the animal. New research, recently published in the Journal of Chromatography B, looks at finding a way to create a highly-sensitive assay to measure the prodrug valacyclovir (VACV) as well as its metabolite, acyclovir (ACV) in both mouse and human plasma samples of limited volume.

Working with Limited Volumes

When it comes to drug discovery and development, pre-clinical pharmacokinetic studies use mouse models most frequently (among animal models). Because a mouse is physically small with a limited volume of biological fluid available, many studies will use composite blood sampling. With this method, a data point is collected from multiple mice, which allows for a sufficient aggregate volume to be collected but can sometimes create complications (like inter-animal variabilities), and requires more animal and material usage in general.

Developing a Highly-Sensitive Assay

To overcome these challenges, the researchers wanted to create a highly-sensitive assay for the target compound that would require a much smaller sample. Not only would this allow for a PK profile from one unique mouse, but would also allow the same strategy to be applied to other studies where the available volume of a blood sample may be limited.

Prior to this study, existing LC-MS/MS methods to quantify VACV and ACV in plasma needed a rather large sample volume (in one such study, the sample volume measured 250ul).

Smaller Sample Volumes Confirmed

The researchers were able to develop and validate a method by which they were able to accurately analyze VACV and ACV in small volumes of mouse plasma, requiring a plasma sample size of only 10ul. This method was applicable to a mouse PK study, and would also make a promising method for human PK studies - especially in circumstances where the sample volume may be a limiting factor - for example, in pediatrics and/or neonatal applications.

Further Reading & References:

A sensitive liquid chromatography-tandem mass spectrometry method for the quantification of valacyclovir and its metabolite acyclovir in mouse and human plasma. Jian Shi, Yongjun Hu, David E. Smith, Hao-Jie Zhu. Journal of Chromatogrpahy B (2018). https://doi.org/10.1016/j.jchromb.2018.06.040


Innovative Research was established in 1998 after the realization that dependable, high-quality, and affordable research materials were hard to come by. Starting with core products like human plasma and serum, Innovative Research has grown to be a trusted supplier of all lab reagents, including human biologicals and ELISA kits. Today, we manufacture and supply over 3,000 high-quality human and animal biologicals including plasma, serum, tissues, and proteins.

Interested in Plasma-Related Research?

You may be interested in these products from Innovative Research:


    Innovative Research was established in 1998 after the realization that dependable, high-quality, and affordable research materials were hard to come by. Starting with core products like human plasma and serum, Innovative Research has grown to be a trusted supplier of all lab reagents, including human biologicals and ELISA kits. Today, we manufacture and supply thousands of high-quality human and animal biologicals including plasma, serum, tissues, and proteins.


Related Posts

It's time to go "Back to School" shopping!
It's time to put together that "back to school" shopping list… Laptop for homework… CH...
Read the Article
Preventing the Progression of Alzheimer's Disease with… Toothpaste and Floss?
New research reinforces the many ways oral health is linked to overall systemic health....
Read the Article
You asked for it! Seriously, you've literally been asking for this.
We get unique product requests every day. From exact collection methods to very specifi...
Read the Article

← Older Post Newer Post →