The Rat Anti Sheep Red Blood Cell (SRBC) IgG ELISA Kit is based on a solid phase enzyme-linked immunosorbent assay (ELISA). The assay uses detergent solubilized SRBC ghosts(2) for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated anti-rat IgG antibodies for detection.. Strip well format. Reagents for up to 96 tests. Test serum or plasma samples are diluted and incubated in the microtiter wells for 45 minutes. The microtiter wells are subsequently washed, and HRP conjugate is added and incubated for 45 minutes. Anti-SRBC IgG molecules are thus sandwiched between immobilized SRBC antigens and the detection antibody conjugate. The wells are then washed to remove unbound HRP-labeled antibodies, and TMB Reagent is added and incubated for 20 minutes at room temperature. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow, and optical density is measured spectrophotometrically at 450 nm. The concentration of anti-SRBC IgG in a test sample is proportional to the optical density and is derived by reference to a standard curve. More Details: Detection Target: Anti-SRBC IgG Reactivity: Rat Range: 6.25-100 u/ml At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results. Please note ¢ this product replaces our old product number IRKTAH3035 (same product, new number).

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