Porcine Myoglobin ELISA KitIPCMBKT
The Porcine Myoglobin ELISA Kit is based on the principle of a solid phase enzyme-linked immunosorbent assay (ELISA). A monoclonal anti-myoglobin antibody is used for solid phase immobilization (on the microtiter wells) and a polyclonal anti- myoglobin antibody conjugated to horseradish peroxidase (HRP) is used for detection.. Strip well format. Reagents for up to 96 tests.
The test sample is allowed to react simultaneously with the two antibodies, and the myoglobin molecules are thus sandwiched between the solid phase and enzyme-linked antibodies. After one hour incubation at room temperature, the wells are washed to remove unbound labeled antibodies. TMB (tetramethyl- benzidine) Reagent is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of Stop Solution, changing the color to yellow. The concentration of myoglobin in the test sample is proportional to the absorbance measured spectrophotometrically at 450 nm.
- Detection Target: Myoglobin
- Reactivity: Porcine
- Range: 0.49-31.25 ng/ml
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Please note – this product replaces our old product number IPGYGKT (same product, new number).
This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.
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