Porcine Deoxyribonuclease Purified Lyophilized
IPCDNASEPFLY20KUPorcine Deoxyribonuclease Purified Lyophilized from Innovative Research Chromatographically purified similarly to the procedure of Bernardi et al., Biochim. Biophys. Acta, 129, 1 (1966). This is a dialyzed, lyophilized powder with a concentration of 12,000 units/mg protein.
Deoxyribonuclease II from porcine spleen has a molecular weight of 38 kDa. The enzyme is a glycoprotein endonuclease with trimeric structure. Optimum pH range is 4.5-5.0 at ionic strength 0.15 M. Deoxyribonuclease II (Acid DNase) hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3’-phosphates. It also acts on p-nitrophenylphosphodiesters at pH 5.6-5.9. Bernardi, Biochem. Biophys. Res. Comm., 17, 573 (1971) describes a three stage degradation of native DNA by DNase II. One unit causes an increase in absorbance at 260 nm of 0.001 per minute at 25°C, pH 4.6 using highly polymerized DNA as substrate.
More Details:
- Source: Porcine Spleen
- Purification: Chromatographically purified
- Storage Conditions: -20°C
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