Porcine Deoxyribonuclease Purified Lyophilized

Porcine Deoxyribonuclease Purified Lyophilized


Innovative Research

  • $418.00

Porcine Deoxyribonuclease Purified Lyophilized from Innovative Research Chromatographically purified similarly to the procedure of Bernardi et al., Biochim. Biophys. Acta, 129, 1 (1966). This is a dialyzed, lyophilized powder. Deoxyribonuclease II from porcine spleen has a molecular weight of 38 kDa. The enzyme is a glycoprotein endonuclease with trimeric structure. Optimum pH range is 4.5-5.0 at ionic strength 0.15 M. Deoxyribonuclease II (Acid DNase) hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3Æ-phosphates. It also acts on p-nitrophenylphosphodiesters at pH 5.6-5.9. Bernardi, Biochem. Biophys. Res. Comm., 17, 573 (1971) describes a three stage degradation of native DNA by DNase II. One unit causes an increase in absorbance at 260 nm of 0.001 per minute at 25░C, pH 4.6 using highly polymerized DNA as substrate. More Details: Source: Porcine Spleen Purification: Chromatographically purified Storage Conditions: -20░C At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.
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This material is sold for in-vitro use only for manufacturing and research. This material is not suitable for human or animal use. While we make every effort to ensure the safety of our products, we recommend handling any biological materials with standard precautions as if capable of spreading infectious disease. The statements herin are offered for informational purposes only to be used solely for your consideration, investigation, and verification.

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