Mouse Insulin-Like Growth Factor II (IGF-2) ELISA Kit
Mouse Insulin-Like Growth Factor II (IGF-2) ELISA Kit is for quantitative detection of mouse IGF-2 in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.
The mouse IGF-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IGF-2 has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGF-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IGF-2 amount of sample captured in plate.
- Detection Target: Natural and recombinant Mouse IGF-2
- Reactivity: Mouse
- Range: 62.5pg/ml-4000pg/ml
- Sensitivity: < 5 pg/ml
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Please note – this product replaces our old product number IRKTAH5373 (same product, new number).