Rat Neurotrophic Tyrosine Kinase Receptor Type 3 (TrkC) ELISA Kit

This Rat Neurotrophic Tyrosine Kinase Receptor Type 3 (TrkC) ELISA Kit from Innovative Research is intended for Quantitative Detection of rat TRKC in cell culture supernates and cell lysates. Strip well format. Reagents for up to 96 tests.

This rat TRKC ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TRKC has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: C32-T429) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TRKC is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat TRKC amount of sample captured in plate.

• Detection Target: Neurotrophic Tyrosine Kinase Receptor Type 3 (TrkC)
• Uniprot ID: Q03351)
• Reactivity: Rat
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 156pg/ml-10000pg/ml
• Sensitivity: <10pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: NTRK3 (Neurotrophic Tyrosine Kinase Receptor Type 3), also known as TRKC, is a protein that in humans is encoded by the NTRK3 gene. By PCR analysis of a somatic cell hybrid panel and by fluorescence in situ hybridization with the cDNA clone, it mapped the NTRK3 gene to 15q24-q25. TRKC, a member of the TRK family of tyrosine protein kinase genes. They found that TRKC is preferentially expressed in the brain; in situ hybridization studies showed transcripts in the hippocampus, cerebral cortex, and the granular cell layer of the cerebellum. By functional studies in HeLa cells, 5 miRNAs regulate the truncated form of NTRK3.; Immunogen sequence: 399