Mouse Transferrin ELISA Kit

Mouse Transferrin ELISA Kit from Innovative Research is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Mouse Transferrin in biological samples. Strip well format. Reagents for up to 96 tests. In this assay the Transferrin present in samples reacts with the antiTransferrin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, antiTransferrin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzymelabeled antibodies form complexes with the previously bound Transferrin . Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3Æ,5,5Æ-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Transferrin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Transferrin in the test sample. The quantity of Transferrin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Detection Target: Transferrin Reactivity: Mouse Range: 3.125 ng/ml - 100 ng/ml Sensitivity: 0.875 ng/ml Storage Conditions: 4C At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.