Human Tumor Necrosis Factor Receptor Superfamily Member 1B (CD120b) ELISA Kit

This Human Tumor Necrosis Factor Receptor Superfamily Member 1B (CD120b) ELISA Kit from Innovative Research is intended for quantitative detection of human soluble TNFsR? in cell culture supernates, serum, plasma (heparin, EDTA, citrate) and urine. Strip well format. Reagents for up to 96 tests.

This human sTNFsR? ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for sTNFsR? has been precoated onto 96-well plates. Standards(NSO, L23-D257) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for sTNFsR? is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human sTNFsR? amount of sample captured in plate.

• Detection Target: Tumor Necrosis Factor Receptor Superfamily Member 1B (CD120b)
• Uniprot ID: P20333)
• Reactivity: Human
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 7.8pg/ml-500pg/ml
• Sensitivity: <2pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Tumor necrosis factor receptor p75(TNF-R p75, or TNF-R2) is a 75-kDa type I transmembrane protein expressed predominantly on cells of hematopoietic lineage. TNF-R p75 belongs to the TNF receptor superfamily characterized by cysteine-rich extracellular regions composed of three to six disulfide-linked domains. The tumor necrosis factor receptor II(TNFRII) gene localizes to 1p36. 2, a genomic region characteristically deleted in neuroblastomas and other malignancies. In addition, TNFRII is the principal mediator of the effects of TNF on cellular immunity, and it may cooperate with TNFRI in the killing of nonlymphoid cells. The standard product used in this kit is recombinant human sTNFRII, consisting of 24-206 amino acid sequence with the molecular mass of 20KDa.; Immunogen sequence: 350