Human Retinol Binding Protein ELISA Kit

Human Retinol Binding Protein ELISA Kit from Innovative Research is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Human Retinol Binding Protein in biological samples. Strip well format. Reagents for up to 96 tests. In this assay the Retinol Binding Protein (RBP) present in samples reacts with the anti-RBP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, antiRBP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzymelabeled antibodies form complexes with the previously bound RBP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3Æ,5,5Æ-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of RBP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of RBP in the test sample. The quantity of RBP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Detection Target: Retinol Binding Protein Reactivity: Human Range: 3.125 ng/ml - 100 ng/ml Sensitivity: 1.163 ng/ml Storage Conditions: 4C At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.