Human Osteopontin ELISA Kit

Human Osteopontin ELISA Kit from Innovative Research is designed for the quantitative determination of Human Osteopontin in plasma and urine samples. Strip well format. Reagents for up to 96 tests.

is a sandwich type immunoassay. The 96-well microplate is coated with a polyclonal antibody specific for human osteopontin. The standards and samples are added to the microplate wells. The microplate is then incubated at room temperature without agitation. After the first incubation is complete, the wells are washed with Wash Buffer and blotted dry. The polyclonal detection antibody conjugate is then added, and the microplate is incubated a second time at room temperature without agitation. After the second incubation is complete, the wells are washed with Wash Buffer and blotted dry. HRP conjugated streptavidin is then added, and the microplate is incubated a third time. After the third incubation is complete, the wells are washed with Wash Buffer and blotted dry. TMB Substrate is added, and the microplate is incubated a fourth time at room temperature without agitation. Once the incubation is complete, Stop Solution is added, and the optical density (OD) is measured by a spectrophotometer at 450 nm. The intensity of the color generated is directly proportional to the amount of human osteopontin in the sample.

• Detection Target: Osteopontin
• Reactivity: Human
• Range: 0.3125 ng/ml - 20 ng/ml
• Sensitivity: 0.510 ng/ml
• Storage Conditions: 4C

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