This Human Junctional Adhesion Molecule-Like ELISA Kit from Innovative Research is intended for quantitative detection of human AMICA/JAML in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.

This human AMICA/JAML ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for AMICA/JAML has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: L20-L275) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for AMICA/JAML is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.

• Detection Target: Junctional Adhesion Molecule-Like
• Uniprot ID: Q86YT9)
• Reactivity: Human
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <10pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. Expression system for standard: JAML or Junctional Adhesion Molecule-Like, or AMICA1 is a JAM transmembrane protein family member. This gene is mapped to 11q23.3. It is composed of two extracellular immunoglobulin-like domains, a membrane-spanning region, and a cytoplasmic tail involved in activation signaling. A known ligand of JAML is Coxsackie virus and Adenovirus Receptor (CXADR in humans and CAR in mice) which has been shown to localize to the tight junctions of epithelial cells. JAML-mediated activation of CAR is required for neutrophil extravasation in addition to other leukocyte/epithelial cell interaction models. Other members of the JAM family of transmembrane proteins include JAM1, JAM2 and JAM3.; Immunogen sequence: 369

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