Human Cathepsin V ELISA Kit

This Human Cathepsin V ELISA Kit from Innovative Research is intended for quantitative detection of human Cathepsin V in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.

This human Cathepsin V ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Cathepsin V has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: V18-V334) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cathepsin V is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.

• Detection Target: Cathepsin V
• Uniprot ID: O60911)
• Reactivity: Human
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 78pg/ml-5000pg/ml
• Sensitivity: <15pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. Expression system for standard: Cathepsin L2, also known as cathepsin V and encoded by the CTSL2 gene, is a human gene. It is mapped to 9q22.33. The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may play an important role in corneal physiology. This gene is expressed in colorectal and breast carcinomas but not in normal colon, mammary gland, or peritumoral tissues, suggesting a possible role for this gene in tumor processes. Alternatively spliced variants, encoding the same protein, have been identified.; Immunogen sequence: 333