Human Bone Morphogenetic Protein 2 ELISA Kit

This Human Bone Morphogenetic Protein 2 ELISA Kit from Innovative Research is intended for quantitative detection of human BMP-2 in bone tissue, cell culture supernates and serum. Strip well format. Reagents for up to 96 tests.

This human BMP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for BMP-2 has been precoated onto 96-well plates. Standards(CHO, Q283-R396) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for BMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human BMP-2 amount of sample captured in plate.

• Detection Target: Bone Morphogenetic Protein 2
• Uniprot ID: P12643)
• Reactivity: Human
• Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <2pg/ml
• Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Additional Information: The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated monoclonal antibody from mouse. Expression system for standard: Bone morphogenetic protein-2(BMP-2, BMP 2A) belongs to the transforming growth factor beta(TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice.1 BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase(MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis.2 Bone morphogenic proteins(BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.3 The standard product used in this kit is recombinant human BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.; Immunogen sequence: 400