Human Beta 2 Microglobulin ELISA Kit

Human Beta 2 Microglobulin ELISA Kit from Innovative Research is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Human Beta 2-Microglobulin (B2M) in biological fluids. Strip well format. Reagents for up to 96 tests. In this assay the Beta 2- Microglobulin present in samples reacts with the antiBeta 2-Microglobulin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-B2M antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound B2M. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3Æ,5,5Æ-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of B2M in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of B2M in the test sample. The quantity of B2M in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Detection Target: Beta 2 Microglobulin Reactivity: Human Range: 3.125 ng/ml - 100 ng/ml Sensitivity: 0.822 ng/ml Storage Conditions: 4C At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.