Bovine Interleukin-6 (IL-6) ELISA Kit

Bovine Interleukin-6 (IL-6) ELISA Kit from Innovative Research is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Bovine Interleukin-6 (IL-6) in biological fluids. Strip well format. Reagents for up to 96 tests. In this assay the IL-6 present in samples reacts with the anti-IL-6 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection antibody, biotin conjugated antiIL-6 is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3Æ,5,5Æ-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IL-6 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IL-6 in the test sample. The quantity of IL-6 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Detection Target: Interleukin-6 (IL-6) Reactivity: Bovine Range: 0.0625 ng/ml - 4 ng/ml Sensitivity: 30 pg/ml Storage Conditions: 4C At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.