*Human Receptor-Associated Protein (RAP) Recombinant Stable MutantIHURAPRSM500UG
Select this product for Molecular Innovations name, Human RAP Stable Mutant and product number, RAP-SM.
Human Receptor-Associated Protein (RAP) Recombinant Stable Mutant from Innovative Research has been recombinantly produced in E. coli and purified by affinity chromatography. This is a Frozen liquid buffered in 0.02M HEPES; 0.15M NaCl; 0.001M CaCl2; pH 7.5 with a concentration of 2.5 mg/ml and a purity of >95% as determined by SDS-PAGE.
- Species: Human
- Target: Receptor-Associated Protein
- Purification Method: Affinity purified
- Purity: >95% as determined by SDS-PAGE Analysis
- Source: E. coli
- Storage Conditions: -70 C
Six mutations were made in the D3 domain (Y260 & T297 changed to cystines, generating a novel disulfide bond between helices 2 & 3; H257, H259, H268, & H290 changed to phenylalines). This is a stable mutuant that is resistent to both pH and heat-induced denaturation. This mutant will not unfold at pH 5.5, has a melting temperature of 73C, and is more resistant to trypsin- and chymotrypsin-mediated proteolysis.
This product can be used as a control in applications like ELISA and Western Blot. It can be used for a wide range of in vitro applications, including blocking, cell binding, cell differentiation, cell signaling, protein-protein interactions, receptor binding, and more. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.
These products are supplied for manufacturing, research and laboratory use only. Researchers and laboratory personnel intending to use any of these products for medical investigation on humans are solely responsible for such use and for compliance with the pertinent regulations of the United States Food and Drug Administration (US-FDA) and other regulations. We do not assume liability for damages resulting from the handling, use and/or disposal of these products, from their use in violation of patent or other rights or reliance upon this information.
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