*Human Pai 1 Recombinant P12 P14 Arginine Double Mutant Substrate

Select this product for Molecular Innovations name, Human PAI-1 (P12 Arg P14 Arg Substrate Mutant) and product number, HPAI-RR.Human PAI-1 Recombinant P12/P14 Arginine Double Mutant Substrate from Innovative Research has been recombinantly produced in E. coli and purified by affinity chromatography. This is a Frozen liquid buffered in 0.05M Sodium Phosphate; 0.1M NaCl; 1mM EDTA; pH 6.6 and a purity of >95% as determined by SDS-PAGE. More Details: Species: Human Target: PAI-1 Purification Method: Affinity purified Purity: >95% as determined by SDS-PAGE Analysis Source: E. coli Storage Conditions: -70 C Additional Information: Two amino acid substituions in the reactive center loop (at P12 and P14) creates a PAI-1 that functions as a substrate for proteinases rather than as an inhibitor. As a result, the reactive center loop will not insert after protease cleavage, and the PAI-1 retains normal vitronectin binding. This double mutation extends the half-life of PAI-1 (as compared to native PAI-1). This product can be used as a control in applications like ELISA and Western Blot. It can be used in a wide range of in vitro applications, including blocking/inhibition, fibrinolysis, plasminogen activation, receptor binding, in hypertension studies, for protein-protein interactions, and more. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.